Coding

Part:BBa_K896906:Design

Designed by: LIAO, WEN-PEN   Group: iGEM12_NYMU-Taipei   (2012-04-26)

this is a hormone produced mainly in guts used for controlling the appetite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 70
    Illegal NheI site found at 93
    Illegal NheI site found at 133
    Illegal NheI site found at 156
    Illegal NheI site found at 196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 802
    Illegal BsaI.rc site found at 283
    Illegal SapI.rc site found at 308


Design Notes

X174_E_FP: gcttctag atggtacgctggactttgt (27nt,TM:55C,%GC:47%)

X174_E_RP: gct ctgcagcggccgctactagta tcactccttccgcacg (40nt,TM:55C,%GC:63%)

Source

sssssssssssssssssssss

References

http://pdf.medrang.co.kr/ekjg/ekjg006-01-02.pdf